<SCRIPT language="javascript">
i = 0;
time = 0;
y1 = 0;
y2 = 0;
y3 = 0;
t = 0.0;
dt = 0.0;
S = 0.0;
E = 0.0;
ES = 0.0;
P = 0.0;
k0 = 0.0;
k1 = 0.0;
k2 = 0.0;
function stop()
{
}
function reStart()
{
i = 0;
time = 0;
y1 = 0;
y2 = 0;
y3 = 0;
t = 0.0; // [seconde]
dt = 0.03; // [seconde]
S = 1000.0; // [umoles/ml]
E = 200.0; // [umoles/ml]
ES = 0.0; // [umoles/ml]
P = 0.0; // [umoles/ml]
k0 = 0.5; // [1/sec]
k1 = 0.0008; // [1/umoles.sec]
k2 = 0.000125; // [1/sec]
time = Math.round(0.0);
y1 = Math.round(S);
y2 = Math.round(ES);
y3 = Math.round(P);
}
function run()
{
document.tellerY1.setValue(Math.round(y1));
document.tellerY2.setValue(Math.round(y2));
document.tellerY3.setValue(Math.round(y3));
document.tellerT.setValue(Math.round(t));
document.kader1.setGrafiek1YT1(time,y1);
document.kader1.setGrafiek2YT1(time,y2);
document.kader2.setGrafiek1YT1(time,y3);
i = i + 1;
t = t + dt;
dPdt = + k0*ES;
P = P + dPdt*dt;
dSdt = - k1*E*S + k2*ES;
S = S + dSdt*dt;
dEdt = - k1*E*S + k2*ES + k0*ES;
E = E + dEdt*dt;
dESdt = + k1*E*S - k2*ES - k0*ES;
ES = ES + dESdt*dt;
time = Math.round(i);
y1 = Math.round(S);
y2 = Math.round(ES);
y3 = Math.round(P);
document.kader1.setGrafiek1YT2(time,y1);
document.kader1.setGrafiek2YT2(time,y2);
document.kader2.setGrafiek1YT2(time,y3);
if (i<200) {window.setTimeout("run()",50)} else {stop()};
}
</SCRIPT>
<BODY onLoad="reStart()">
<CENTER>
<FORM>
<H1>Enzymkinetics </H1>
<H3>(an example of a model-driven simulation with animation)</H3>
<B>S</B> + <B>E</B> <==k1/k2==> <B>ES</B> ---k0---> <B>P</B>
<P>
<APPLET
CODE="Animatie.class"
NAME='pS'
width="25"
height="200">
</APPLET>
<APPLET
CODE="grafiekYT.class"
NAME='kader1'
width=160
height=200>
<param name="BackColor" value="230,230,230">
<param name="GridColor" value="0,0,0">
<param name="Graph1Color" value="255,0,0">
<param name="Graph2Color" value="0,0,255">
<param name="GridT" value="3">
<param name="GridY" value="10">
<param name="Y1min" Value="0">
<param name="Y1max" Value="1000">
<param name="Y1text" Value="Substrate [S]">
<param name="Y2min" Value="0">
<param name="Y2max" Value="1000">
<param name="Y2text" Value="ES-complex [ES]">
<param name="Tmin" Value="0">
<param name="Tmax" Value="500"> // 500 punten?
</APPLET>
<APPLET
CODE="Animatie.class"
NAME='aES'
width="25"
height="200">
</APPLET>
<APPLET
CODE="grafiekYT.class"
NAME='kader2'
width=160
height=200>
<param name="BackColor" value="230,230,230">
<param name="GridColor" value="0,0,0">
<param name="Graph1Color" value="255,0,0">
<param name="Graph2Color" value="0,0,255">
<param name="GridT" value="3">
<param name="GridY" value="10">
<param name="Y1min" Value="0">
<param name="Y1max" Value="1000">
<param name="Y1text" Value="Product [P]">
<param name="Y2min" Value="0">
<param name="Y2max" Value="1000">
<param name="Y2text" Value="xx">
<param name="Tmin" Value="0">
<param name="Tmax" Value="500"> //500 punten?
</APPLET>
<APPLET
CODE="Animatie.class"
NAME='aP'
width="25"
height="200">
</APPLET>
<BR>
Tmin: 0 ----------
<INPUT type="button" name="xxxxxx" value="run" onClick="run ()">
<FONT SIZE=-2>time</FONT>
<APPLET
CODE="Teller.class"
NAME='tellerT'
width="50"
height="20">
<param name="FontFamily" value="Courier">
<param name="FontSize" value="30">
<param name="BGCol" value="ff0000">
<param name="StartValue" value="0">
</APPLET>
--> ---------- Tmax: 15 [Sec] <FONT SIZE=-2>(=500*0.03)</FONT><P><B>S</B>
<APPLET
CODE="Teller.class"
NAME='tellerY1'
width="50"
height="20">
<param name="FontFamily" value="Courier">
<param name="FontSize" value="30">
<param name="BGCol" value="ff0000">
<param name="StartValue" value="15">
</APPLET>
<FONT SIZE=-2>(Red)</FONT> ---------- <B>ES</B>
<APPLET
CODE="Teller.class"
NAME='tellerY2'
width="50"
height="20">
<param name="FontFamily" value="Courier">
<param name="FontSize" value="30">
<param name="BGCol" value="ff0000">
<param name="StartValue" value="0">
</APPLET>
<FONT SIZE=-2>(Blue)</FONT> ---------- <B>P</B>
<APPLET
CODE="Teller.class"
NAME='tellerY3'
width="50"
height="20">
<param name="FontFamily" value="Courier">
<param name="FontSize" value="30">
<param name="BGCol" value="ff0000">
<param name="StartValue" value="35">
</APPLET> <FONT SIZE=-2>(Red)</FONT>
</CENTER>
</FORM>
Enschede, Dec, 8, 2003